Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

GABA and benzodiazepine receptors in the gerbil brain after transient ischemia: demonstration by quantitative receptor autoradiography

Journal Article · · J. Cereb. Blood Flow Metab.; (United States)
; ;
Quantitative receptor autoradiography was used to measure the binding of gamma-aminobutyric acid (GABA) and benzodiazepine receptors after ischemia by means of transient occlusion of bilateral common carotid arteries in the gerbil. (/sup 3/H)Muscimol was used to label the GABAA receptors and (/sup 3/H)flunitrazepam to label central type benzodiazepine receptors. In the superolateral convexities of the frontal cortices, (/sup 3/H)muscimol binding was increased in 60% of the animals killed 3 days after ischemia, and decreased in 67% of the animals killed 27 days after ischemia. Twenty-seven days after ischemia, (/sup 3/H)flunitrazepam binding in the substantia nigra pars reticulata increased to 252% of the control, though the increase in (/sup 3/H)muscimol binding was not significant. In the dorsolateral region of the caudate putamen, marked neuronal necrosis and depletion of both (/sup 3/H)muscimol and (/sup 3/H)flunitrazepam binding sites were observed 27 days after ischemia, the ventromedial region being left intact. In spite of the depletion of pyramidal cells in the CA1 region of the hippocampus, both (/sup 3/H)muscimol and (/sup 3/H)flunitrazepam binding sites were preserved 27 days after ischemia. Since our previous study revealed that adenosine A1 binding sites were depleted in the CA1 subfield of the hippocampus after ischemia correlating with neuronal damage, GABAA and benzodiazepine receptors may not be distributed predominantly on the pyramidal cells in the CA1 region.
Research Organization:
Tohoku Univ. School of Medicine, Sendai, Japan
OSTI ID:
6696229
Journal Information:
J. Cereb. Blood Flow Metab.; (United States), Journal Name: J. Cereb. Blood Flow Metab.; (United States) Vol. 1; ISSN JCBMD
Country of Publication:
United States
Language:
English

Similar Records

The GABAA receptor complex in hepatic encephalopathy. Autoradiographic evidence for the presence of elevated levels of a benzodiazepine receptor ligand
Journal Article · Wed Jan 31 23:00:00 EST 1990 · Neuropsychopharmacology; (USA) · OSTI ID:7138041
Functional modulation of cerebral gamma-aminobutyric acidA receptor/benzodiazepine receptor/chloride ion channel complex with ethyl beta-carboline-3-carboxylate: Presence of independent binding site for ethyl beta-carboline-3-carboxylate
Journal Article · Tue May 01 00:00:00 EDT 1990 · Journal of Pharmacology and Experimental Therapeutics; (USA) · OSTI ID:6727847
Benzodiazepine agonist and inverse agonist actions on GABAA receptor-operated chloride channels. II. Chronic effects of ethanol
Journal Article · Tue May 01 00:00:00 EDT 1990 · Journal of Pharmacology and Experimental Therapeutics; (USA) · OSTI ID:6721290

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AMINOBUTYRIC ACID
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
BODY
BRAIN
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DISEASES
DRUGS
HIPPOCAMPUS
ISCHEMIA
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIORECEPTOR ASSAY
RECEPTORS
RODENTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VASCULAR DISEASES
VERTEBRATES