Deletion of chromosome 2 is an early event in the development of radiation-induced myeloid leukemia in SJL/J mice

Journal Article · Mon Aug 01 04:00:00 EDT 1988 · Leukemia; (United States)

OSTI ID:6729116

Trakhtenbrot, L; Krauthgamer, R; Resnitzky, P; Haran-Ghera, N

In this study we have analyzed the chromosomal changes in the preleukemic phase in SJL/J mice treated with radiation and acute myeloid leukemias (AMLs) induced by radiation alone or with additional corticosteroid treatment. SJL/J mice exposed to 300 rad whole body irradiation developed a low incidence of AML (20-25%) that could be markedly increased (to 50-70%) by additional coleukemogenic treatment with corticosteroids. Partial deletion in one chromosome 2 was found in 100% of bone marrow and spleen cells of leukemic animals in both treatment modalities, whereas the age-matched controls exhibited a normal karyotype. Five types of deletion were observed according to site and size, but region D through G was the common missing part in all five types of chromosome 2 deletion. The occurrence of chromosome 2 deletion was also tested among bone marrow cells removed from 17 mice, 4 months after exposure to 300 rad whole body irradiation, long before the time when AML development is expected. About 80% of the mice tested had different levels of deleted chromosome 2 among their bone marrow population. Cytological and histological examination of bone marrow and spleen of most tested animals showed a normal hematologic picture. These results suggest that the marker chromosome is related to the process of radiation-induced initiation of AML in SJL/J mice.

• Research Organization: Weizmann Institute of Science, Rehovot (Israel)
• OSTI ID: 6729116
• Journal Information: Leukemia; (United States), Journal Name: Leukemia; (United States) Vol. 2:8; ISSN LEUKE
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADRENAL HORMONES
ANIMAL CELLS
ANIMALS
BONE MARROW CELLS
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
DISEASES
EXTERNAL IRRADIATION
HEMIC DISEASES
HYDROXY COMPOUNDS
IMMUNE SYSTEM DISEASES
IRRADIATION
KARYOTYPE
KETONES
LEUKEMIA
MAMMALS
MICE
MUTATIONS
MYELOID LEUKEMIA
NEOPLASMS
ORGANIC COMPOUNDS
PREGNANES
RADIOINDUCTION
RODENTS
SOMATIC CELLS
SPLEEN CELLS
STEROIDS
VERTEBRATES
WHOLE-BODY IRRADIATION