Column chromatography separation of polychlorinated biphenyls from dichlorodiphenyltrichloroethane and methabolites

Journal Article · Sat Nov 01 04:00:00 EST 1980 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac50063a060· OSTI ID:6724189

Needham, L L; Smrek, A L; Head, S L; Burse, V W; Liddle, J A

Gas chromatographic determinations of organochlorine insectides or polychlorinated biphenyls (PCBs) in biological material are often complicated by their simultaneous presence in the eluates from the column chromatography steps. A gas chromatography/electron capture detector procedure to analyze serum for PCBs (Aroclor 1260) in the presence of the ortho, para and para, para isomers of dichlorodiphenyltrichloroethane (DDT) and its metabolites, dichlorodiphenyldichloroethylene (DDE) and dichlorodiphenyldichloroethane (DDD) was devised. The use of silver nitrate on silica gel column to separate PCBs from DDT and metabolites are described. Bovine serum was analyzed for DDt, DDE, and DDD. 500 individuals in an Alabama community were studied, the highest concentrations in (ppB) for PCB was 155. The method described yield high recoveries of PCBs, with no interference from chlorinated hydrocarbons normally found in human serum. 2 figures, 2 tables. (DP)

• Research Organization: Dept. of Health and Human Services, Atlanta, GA
• OSTI ID: 6724189
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 52:13; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
AROMATICS
BIPHENYL
CHLORINATED AROMATIC HYDROCARBONS
CHROMATOGRAPHY
DDT
ELECTRON-CAPTURE DETECTORS
GAS CHROMATOGRAPHY
HALOGENATED AROMATIC HYDROCARBONS
HYDROCARBONS
INSECTICIDES
MEASURING INSTRUMENTS
METABOLITES
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PESTICIDES
RADIOMETRIC GAGES
SEPARATION PROCESSES