Development of more efficacious Tc-99m organ imaging agents for use in nuclear medicine by analytical characterization of radiopharmaceutical mixtures. Progress report, May 1, 1980-April 30, 1981. [/sup 99m/Tc(NaBH/sub 4/)HEDP]
High performance liquid chromatography (HPLC) was used to efficiently separate and detect the individual components in a radiopharmaceutical mixture. A procedure for separating Tc(NaBH/sub 4/)HEDP radiopharmaceutical analogues by anion exchange HPLC with uv-visible and ..gamma.. detection was developed. Such preparations consist of as many as 7 Tc-containing components, the relative quantities of which are highly dependent on the conditions during preparation and subsequent handling. The in vivo distributions of 3 of the isolated Tc-HEDP species were evaluated as skeletal imaging agents in normal rats. The chromatographically separated Tc-HEDP components exhibit distinctly different biodistributions which are related to the chromatographic characteristics of the individual components. These same Tc-HEDP components were evaluated for uptake in myocardial infarcts using the isoproterenol-induced necrosis model in the rat. The relative uptake of the various components in the infarcted heart parallels the skeletal uptake. An optically transparent thin layer electrochemical flow cell was developed and characterized. This cell enables optical and electrochemical measurements to be made simultaneously on individual Tc-HEDP complexes as they elute from the HPLC. These results demonstrate the potential presence of numerous technetium complexes in radiopharmaceuticals and the efficacy of HPLC as a mode of separation and detection of these complexes. One particular component in the Tc(NaBH/sub 4/)HEDP radiopharmaceutical analogue is more effective than the others for both skeletal and myocardial infarct uptake. This is strongly suggestive that a more efficacious radiopharmaceutical would result from the administration of this single component.
- Research Organization:
- Cincinnati Univ., OH (USA)
- Sponsoring Organization:
- USDOE Office of Environment, Safety and Health (EH)
- DOE Contract Number:
- AC02-80EV10380
- OSTI ID:
- 6723475
- Report Number(s):
- DOE/EV/10380-1; TRN: 81-002627
- Country of Publication:
- United States
- Language:
- English
Similar Records
Development of more efficacious Tc-99m organ imaging agents for use in nuclear medicine by analytical characterization of radiopharmaceutical mixtures. Progress report, May 1, 1982-April 30, 1983
Development of more efficacious TC-99M organ imaging agents for use in nuclear medicine by analytical characterization of radiopharmaceutical mixtures. Progress report, May 1, 1983-April 1, 1984
Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
MYOCARDIUM
RADIONUCLIDE KINETICS
SCINTISCANNING
PHOSPHONIC ACID ESTERS
RADIOPHARMACEUTICALS
ION EXCHANGE CHROMATOGRAPHY
LIQUID COLUMN CHROMATOGRAPHY
TISSUE DISTRIBUTION
SKELETON
TECHNETIUM 99
BIOLOGICAL LOCALIZATION
BOROHYDRIDES
COMPARATIVE EVALUATIONS
CORRELATIONS
ELECTROCHEMICAL CELLS
FEASIBILITY STUDIES
ISOMERIC NUCLEI
QUALITATIVE CHEMICAL ANALYSIS
RATS
SODIUM COMPOUNDS
ALKALI METAL COMPOUNDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BORON COMPOUNDS
CARDIOVASCULAR SYSTEM
CHEMICAL ANALYSIS
CHROMATOGRAPHY
COUNTING TECHNIQUES
DIAGNOSTIC TECHNIQUES
DISTRIBUTION
DRUGS
ESTERS
HEART
HOURS LIVING RADIOISOTOPES
HYDROGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MUSCLES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
RADIOACTIVE MATERIALS
RADIOISOTOPE SCANNING
RADIOISOTOPES
RODENTS
SEPARATION PROCESSES
TECHNETIUM ISOTOPES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
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