Development of more efficacious Tc-99m organ imaging agents for use in nuclear medicine by analytical characterization of radiopharmaceutical mixtures. Progress report, May 1, 1980-April 30, 1981. [/sup 99m/Tc(NaBH/sub 4/)HEDP]

Technical Report · Mon Dec 01 04:00:00 EST 1980

DOI:https://doi.org/10.2172/6723475· OSTI ID:6723475

Heineman, W. R.; Deutsch, E. A.

High performance liquid chromatography (HPLC) was used to efficiently separate and detect the individual components in a radiopharmaceutical mixture. A procedure for separating Tc(NaBH/sub 4/)HEDP radiopharmaceutical analogues by anion exchange HPLC with uv-visible and ..gamma.. detection was developed. Such preparations consist of as many as 7 Tc-containing components, the relative quantities of which are highly dependent on the conditions during preparation and subsequent handling. The in vivo distributions of 3 of the isolated Tc-HEDP species were evaluated as skeletal imaging agents in normal rats. The chromatographically separated Tc-HEDP components exhibit distinctly different biodistributions which are related to the chromatographic characteristics of the individual components. These same Tc-HEDP components were evaluated for uptake in myocardial infarcts using the isoproterenol-induced necrosis model in the rat. The relative uptake of the various components in the infarcted heart parallels the skeletal uptake. An optically transparent thin layer electrochemical flow cell was developed and characterized. This cell enables optical and electrochemical measurements to be made simultaneously on individual Tc-HEDP complexes as they elute from the HPLC. These results demonstrate the potential presence of numerous technetium complexes in radiopharmaceuticals and the efficacy of HPLC as a mode of separation and detection of these complexes. One particular component in the Tc(NaBH/sub 4/)HEDP radiopharmaceutical analogue is more effective than the others for both skeletal and myocardial infarct uptake. This is strongly suggestive that a more efficacious radiopharmaceutical would result from the administration of this single component.

• Research Organization: Cincinnati Univ., OH (USA)
• Sponsoring Organization: USDOE Office of Environment, Safety and Health (EH)
• DOE Contract Number: AC02-80EV10380
• OSTI ID: 6723475
• Report Number(s): DOE/EV/10380--1
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
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560172 -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Animals-- (-1987)
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL COMPOUNDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BODY
BOROHYDRIDES
BORON COMPOUNDS
CARDIOVASCULAR SYSTEM
CHEMICAL ANALYSIS
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
CORRELATIONS
COUNTING TECHNIQUES
DIAGNOSTIC TECHNIQUES
DISTRIBUTION
DRUGS
ELECTROCHEMICAL CELLS
ESTERS
FEASIBILITY STUDIES
HEART
HOURS LIVING RADIOISOTOPES
HYDROGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
ION EXCHANGE CHROMATOGRAPHY
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MATERIALS
MUSCLES
MYOCARDIUM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHONIC ACID ESTERS
QUALITATIVE CHEMICAL ANALYSIS
RADIOACTIVE MATERIALS
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIONUCLIDE KINETICS
RADIOPHARMACEUTICALS
RATS
RODENTS
SCINTISCANNING
SEPARATION PROCESSES
SKELETON
SODIUM COMPOUNDS
TECHNETIUM 99
TECHNETIUM ISOTOPES
TISSUE DISTRIBUTION
VERTEBRATES
YEARS LIVING RADIOISOTOPES