Effects of NaCl on proline synthesis and utilization in excised barley leaves
Proline accumulation in NaCl-treated excised barley leaves was studied. Leaves were treated by placing the cut end in NaCl solutions and allowing the salt to enter the leaf via the transpiration stream. Leaves treated this way maintained turgor while the sodium content increased and the osmotic potential decreased. Proline began accumulating after 12 hours and continued accumulating over the subsequent 12-hour period at an average rate of 0.6 micromoles per hour per gram fresh weight. During the time proline was accumulating, (/sup 14/C)glutamate was added to measure the effects of salt on proline synthesis from glutamate and (/sup 14/C)proline was added in separate experiments to determine the effect of salt on proline utilization. Salt treatment dramatically increased proline synthesis from glutamate. Proline utilization by oxidation and for protein synthesis was decreased by 50 and 60%, respectively, by the salt treatment. These effects are similar to the effects of drought and abscisic acid in barley leaves. The results indicate that common mechanisms cause proline to accumulate under these different stresses. 12 references, 5 figures. 1 table.
- Research Organization:
- Iowa State Univ., Ames
- DOE Contract Number:
- AC02-82ER12028
- OSTI ID:
- 6721379
- Journal Information:
- Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 72; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
AMINES
AMINO ACIDS
AZOLES
BIOLOGICAL ACCUMULATION
BIOLOGICAL EFFECTS
BIOLOGICAL STRESS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHLORIDES
CHLORINE COMPOUNDS
DATA
EXPERIMENTAL DATA
GLUTAMIC ACID
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEAVES
METABOLISM
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROLINE
PYRROLES
PYRROLIDINES
SODIUM CHLORIDES
SODIUM COMPOUNDS
TRACER TECHNIQUES
TRANSPIRATION