Steady state proline levels in salt-shocked barley leaves
Excised barley leaves were treated with salt solutions or wilted. After the treatment period, the leaves were allowed to recover in a 50 millimolar sucrose and 1 millimolar glutamate solution, and proline, Na/sup +/, and K/sup +/ were measured at intervals. Na/sup +/ and K/sup +/ concentrations stayed at a constant high level after the salt treatments, and proline increased to a steady state concentration in response. the relationship between the maximum rate of proline accumulation and the Na/sup +/ concentration reached in each experiment was linear. The final steady state proline concentration reached was also directly proportional to the Na/sup +/ concentration. For a given Na/sup +/ concentration in the leaves, the steady state proline level was greater when 410 millimolar NaCl was added to the leaves than when 205 millimolar NaCl was added. These results are consistent with proline acting as a compatible cytoplasmic solute, balancing an accumulation of salts outside of the cytoplasm. In contrast to the proline levels in salt-shocked leaves, the concentrations in wilted leaves decreased to near control levels within 24 hours of relief of stress. 20 references, 4 figures.
- Research Organization:
- Iowa State Univ., Ames
- DOE Contract Number:
- AC02-82ER12028
- OSTI ID:
- 6702899
- Journal Information:
- Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 76; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
AMINES
AMINO ACIDS
AZOLES
BARLEY
BIOLOGICAL ACCUMULATION
BIOLOGICAL STRESS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CEREALS
CYTOPLASM
DATA
EXPERIMENTAL DATA
GRASS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INFORMATION
LEAVES
METABOLISM
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
POTASSIUM COMPOUNDS
PROLINE
PYRROLES
PYRROLIDINES
SODIUM COMPOUNDS
STEADY-STATE CONDITIONS