Relationship between stress-induced ABA and proline accumulations and ABA-induced proline accumulation in excised barley leaves

Journal Article · Sun Sep 01 04:00:00 EDT 1985 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.79.1.24· OSTI ID:6024681

Stewart, C R; Voetberg, G

When excised second leaves from 2-week-old barley (Hordeum vulgare var Larker) plants were incubated in a wilted condition, abscisic acid (ABA) levels increased to 0.6 nanomole per gram fresh weight at 4 hours then declined to about 0.3 nanomole per gram fresh weight and remained at that level until rehydrated. Proline levels began to increase at about 4 hours and continued to increase as long as the ABA levels were 0.3 nanomole per gram fresh weight or greater. Upon rehydration, proline levels declined when the ABA levels fell below 0.3 nanomole per gram fresh weight. Proline accumulation was induced in turgid barley leaves by ABA addition. The threshold level of ABA for proline accumulation appeared to be similar for wilted leaves where ABA increased endogenously and for turgid leaves where ABA was added exogenously. However, the rate of proline accumulation was more dependent on ABA levels in turgid leaves to which ABA was added exogenously than in wilted leaves. Salt-induced proline accumulation was not preceded by increases in ABA-levels comparable to those observed in wilted leaves. 17 references, 4 figures.

• Research Organization: Iowa State Univ., Ames
• DOE Contract Number: AC02-82ER12028
• OSTI ID: 6024681
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 79:1; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

551000* -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
ABSCISIC ACID
AMINES
AMINO ACIDS
AUXINS
AZOLES
BARLEY
BIOLOGICAL STRESS
CARBOXYLIC ACIDS
CEREALS
DOSE-RESPONSE RELATIONSHIPS
GRASS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
LEAVES
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYSIOLOGY
PLANT GROWTH REGULATORS
PLANTS
PROLINE
PYRROLES
PYRROLIDINES
UPTAKE