Atrial natriuretic peptide increases microvascular blood flow and macromolecular escape during renin infusion in the hamster

Journal Article · Thu Feb 01 04:00:00 EST 1990 · Microcirculation, Endothelium, and Lymphatics; (USA)

OSTI ID:6718694

Boric, M P; Albertini, R ^[1]

---

1. P. Universidad Catolica de Chile, Santiago (Chile)

The effects of Atrial Natriuretic Peptide (ANP) on microvascular hemodynamics and macromolecular permselectivity were studied in the hamster cheek pouch under resting conditions and during intravenous renin infusion. Fluorescent intravital microscopy was used to observe arteriolar diameters and to detect escape of fluorescent dextran of 150 K-Daltons (FITC-Dx-150). Microvascular plasma flow was estimated by clearance of 51Cr-EDTA and net macromolecular transport by clearance of FITC-Dx-150. At rest, topical ANP (2-250 ng/ml) had no effect on arteriolar diameter, 51Cr-EDTA clearance, relative vascular conductance (RVC) or FITC-Dx-150 clearance. Infusion of renin (10 mU/Kg/Hr, iv) elevated systemic arterial pressure by 30% and reduced cheek pouch RVC by 26%. During renin infusion, topical ANP (50 ng/ml) produced transient arteriolar vasodilation, and increased 51Cr-EDTA clearance (+35%), RVC (+58%) and FITC-Dx-150 clearance (+54%), without affecting systemic pressure. ANP did not induce venular leakage sites under any condition, but changes in FITC-Dx-150 clearance were highly correlated with changes in 51Cr-EDTA clearance, suggesting that the larger macromolecular escape was due to increases in microvascular blood flow and capillary/post-capillary hydrostatic pressure.

• OSTI ID: 6718694
• Journal Information: Microcirculation, Endothelium, and Lymphatics; (USA), Journal Name: Microcirculation, Endothelium, and Lymphatics; (USA) Vol. 6:1; ISSN MELYE; ISSN 0740-9451
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
AROMATICS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BLOOD FLOW
BLOOD PRESSURE
BLOOD SUBSTITUTES
BLOOD VESSELS
BLOOD-PLASMA CLEARANCE
BODY
CAPILLARIES
CARBOHYDRATES
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CHELATING AGENTS
CHROMIUM 51
CHROMIUM ISOTOPES
CLEARANCE
DEXTRAN
DRUGS
DYES
DYNAMIC FUNCTION STUDIES
EDTA
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
FLUORESCEIN
FLUORESCENCE
HAMSTERS
HEMATOLOGIC AGENTS
HYDROLASES
HYDROXY ACIDS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPES
LUMINESCENCE
MAMMALS
MEMBRANE TRANSPORT
NONSPECIFIC PEPTIDASES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PEPTIDES
PERMEABILITY
PHENOLS
POLYPHENOLS
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
RENIN
RODENTS
SACCHARIDES
VASODILATION
VERTEBRATES