Nucleotide sequence of the gene encoding the nitrogenase iron protein of Thiobacillus ferrooxidans
The DNA sequence was determined for the cloned Thiobacillus ferrooxidans nifH and part of the nifD genes. The DNA chains were radiolabeled with (..cap alpha..-/sup 32/P)dCTP (3000 Ci/mmol) or (..cap alpha..-/sup 35/S)dCTP (400 Ci/mmol). A putative T. ferrooxidans nifH promoter was identified whose sequences showed perfect consensus with those of the Klebsiella pneumoniae nif promoter. Two putative consensus upstream activator sequences were also identified. The amino acid sequence was deduced from the DNA sequence. In a comparison of nifH DNA sequences from T. ferrooxidans and eight other nitrogen-fixing microbes, a Rhizobium sp. isolated from Parasponia andersonii showed the greatest homology (74%) and Clostridium pasteurianum (nifH1) showed the least homology (54%). In the comparison of the amino acid sequences of the Fe proteins, the Rhizobium sp. and Rhizobium japonicum showed the greatest homology (both 86%) and C. pasteurianum (nifH1 gene product) demonstrated the least homology (56%) to the T. ferrooxidans Fe protein.
- Research Organization:
- Univ. of Cape Town, Rondebosch, South Africa
- OSTI ID:
- 6712741
- Journal Information:
- J. Bacteriol.; (United States), Vol. 169:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
NITROGENASE
AMINO ACID SEQUENCE
THIOBACILLUS FERROXIDANS
DNA SEQUENCING
CLOSTRIDIUM
COMPARATIVE EVALUATIONS
MOLECULAR BIOLOGY
PHOSPHORUS 32
RHIZOBIUM
SULFUR 35
TRACER TECHNIQUES
BACILLUS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
MOLECULAR STRUCTURE
NITRO-GROUP DEHYDROGENASES
NUCLEI
ODD-ODD NUCLEI
OXIDOREDUCTASES
PHOSPHORUS ISOTOPES
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
SULFUR ISOTOPES
SULFUR-OXIDIZING BACTERIA
550401* - Genetics- Tracer Techniques