beta. -adrenergic ((/sup 3/H) CGP-12177) receptors are elevated in slices of soleus muscle from CHF 147 dystrophic hamsters

Journal Article · Mon Mar 23 04:00:00 EST 1987 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(87)90235-9· OSTI ID:6710585

Watson-Wright, W M; Wilkinson, M

The authors utilized a muscle slice technique to compare the ontogeny of cell surface ..beta..-adrenergic receptor binding in soleus and extensor digitorum longus (EDL) muscles of male Golden Syrian (GS) and Canadian Hybrid Farms 147 (CHF 147) dystrophic hamsters. Binding of the ..beta..-adrenergic antagonist, (/sup 3/H) CGP-12177 (CGP), to GS muscle slices was reversible, saturable, stereospecific and of high affinity. Bmax was higher in the soleus (2.57+/-.12 fmol/mg wet wt) than in the EDL (1.61+/-.17 fmol/mg wet wt) of adult animals while affinities were similar (0.35+/-.06 and 0.24+/-.04 nM respectively). No differences in binding characteristics were seen in EDL of GS compared to CHF 147 animals. In soleus slices from GS hamsters, Bmax was highest at 16 days of age (5.72+/-0.26 fmol/mg), decreased between 16 and 29 days and remained constant until 300 days (2.51+/-0.52 fmol/mg). In dystrophic soleus slices, Bmax was also higher at 16 days than at any other age but receptor number decreased gradually, remaining higher than in GS until 90 days of age (p<0.05). The failure of ..beta..-adrenergic receptor number to decrease at a normal rate may be implicated in the pathogenesis of hamster polymyopathy. 21 references, 5 figures, 1 table.

• Research Organization: Dalhousie Univ., Halifax, Nova Scotia
• OSTI ID: 6710585
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 40:12; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
DISEASES
DRUGS
HAMSTERS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MUSCLES
NERVOUS SYSTEM DISEASES
ONTOGENESIS
ORGANIC COMPOUNDS
PATHOGENESIS
PROTEINS
REACTION KINETICS
RECEPTORS
RODENTS
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES