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beta. -adrenergic receptor binding characteristics and responsiveness in cultured Wistar-Kyoto rat arterial smooth muscle cells

Journal Article · · Life Sci.; (United States)
;
The tone of arterial blood vessels is regulated by the catecholamines through their receptors on arterial smooth muscle cells (ASMC). ..beta..-/sub 2/-adrenergic receptors of ASMC mediate vasodilation through agonist mediated c-AMP production. Previous reports have described these receptors on freshly isolated blood vessels. This study demonstrates the presence of ..beta../sub 2/-adrenergic receptors on cultured rat ASMC and that these receptors are functional. ..beta..-adrenergic receptor binding was measured using (/sup 3/H)-dihydroalprenolol (DHA) binding to the membrane of cultured ASMC from normotensive Wistar-Kyoto rats. The ASMC ..beta..-adrenergic receptors have a Kd of 0.56 +/- 0.16 nM and a Bmax of 57.2 +/- 21.7 fmol/mg protein. Competition binding studies revealed a much greater affinity of these receptors for epinephrine than norepinephrine, indicating the preponderance of a ..beta../sub 2/-adrenergic receptor subtype. Isoproterenol stimulation of cultured ASMC resulted in a 14 +/- 7 fold increase in intracellular c-AMP content of these cells indicating these receptors are functional. ..beta..-adrenergic receptors of cultured ASMC provide an excellent system in which the association between hypertension and observed ..beta..-adrenergic receptor differences can be further explored.
Research Organization:
Univ. of Texas Medical Branch, Galveston (USA)
OSTI ID:
6152566
Journal Information:
Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 43:8; ISSN LIFSA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMP
ANIMALS
ARTERIES
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BLOOD VESSELS
BODY
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
CHEMICAL PROPERTIES
DISEASES
DRUGS
HYPERTENSION
KINETICS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
MUSCLES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SYMPATHOMIMETICS
SYMPTOMS
VASCULAR DISEASES
VASODILATION
VERTEBRATES