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Nuclear magnetic resonance studies of biological molecules

Thesis/Dissertation ·
OSTI ID:6709745

A number of biological systems are investigated using a variety of nuclear magnetic resonance (NMR) techniques. The first two studies are applications of the technique to systems in solution. Proton NMR and lanthanide shift agents are used to determine the outside to inside ratio of lipids in large unilamellar vesicles produced by the method of reverse-phase evaporation. The observed ratio of 1.1 is indicative of the unilamellar nature of the vesicles. In order to facilitate the study of motions of the carbohydrate moiety of glycoproteins, a method of enzymatically attaching a carbon-13 (/sup 13/C) enriched galactopyranose onto the nonreducing end of the carbohydrate chain has been developed. The overall reaction is followed using /sup 31/P and /sup 13/C NMR relaxation parameters permits the modeling of motions characteristic of the attached galactose residue. The third study is an application of the technique to live animals. Non-invasive, real time observation of changes in brain energy phosphates in young, middle age and old rats taken into and recovered from mild hypoxic stress has been accomplished in vivo with phosphorus-31 NMR. Age related changes in brain oxidative metabolism apparently are manifest significantly only under physiological or pharmacological stress. Each age group displayed somewhat different patterns of phosphate metabolite alterations during the time course of the hypoxic episode and subsequent recovery.

Research Organization:
California Univ., San Francisco (USA)
OSTI ID:
6709745
Country of Publication:
United States
Language:
English

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Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMALS
BODY
BRAIN
CARBOHYDRATES
CARBON 13
CARBON ISOTOPES
CENTRAL NERVOUS SYSTEM
EVEN-ODD NUCLEI
GALACTOSE
GLUCOPROTEINS
HEXOSES
IN VIVO
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
LIPIDS
MAGNETIC RESONANCE
MAMMALS
METABOLISM
MONOSACCHARIDES
NERVOUS SYSTEM
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS 31
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PROTEINS
RATS
RESONANCE
RODENTS
SACCHARIDES
STABLE ISOTOPES
TRACER TECHNIQUES
VERTEBRATES