Dolichol phosphate induces non-bilayer structures, vesicle fusion and transbilayer movements of lipids in model membranes

Conference · Fri May 01 04:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6416825

de Kruijff, B; Van Duijn, G; Valtersson, C; Chojnacki, T; Verkleij, A J; Dallner, G

The effect of dolichols, polyprenols, dolichol esterified with fatty acids, and dolichol phosphate on the structure and fluidity of model membranes was studied using different biophysical techniques. These studies suggest that (1) dolichol and dolichol derivatives destabilize unsaturated PE-containing bilayers and promote hexagonal II phase formation; (2) high concentrations of dolichol induce lipid structures characterized by isotropic T P NMR and particulate fracture faces. The effect of dolichol and dolichyl phosphate on fusion between large unilamellar vesicles of DOPC and DOPE was studied using a fluroescence resonance energy transfer assay. The influence of dolichyl phosphate on the transbilary movement of DOPC in multilamellar vesicles (MLV) and large unilamellar vesicles (LUV) composed of DOPC and DOPE (1:2) was investigated by using the PC-specified transfer protein. The results indicate that: (1) both dolichol and dolichyl phosphate enhance vesicle fusion in a comparable and concentration-dependent way; (2) the amount of exchangeable PC from MLVs is increased by dolichyl phosphate probably as a result of fusion processes. It thus appears that these polyprenols are potent destabilizers of bilayer structure and that this process is accompanied by membrane fusion and transbilayer transport of phospholipids.

• Research Organization: Univ. of Utrecht, Netherlands
• OSTI ID: 6416825
• Report Number(s): CONF-870644-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL MODELS
CELL CONSTITUENTS
CELL MEMBRANES
DOSE-RESPONSE RELATIONSHIPS
ISOTOPES
LIGHT NUCLEI
LIPIDS
MEMBRANE TRANSPORT
MEMBRANES
MOLECULAR STRUCTURE
NMR SPECTRA
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 31
PHOSPHORUS ISOTOPES
RESPONSE MODIFYING FACTORS
SPECTRA
STABLE ISOTOPES