Complete structure of the cell surface polysaccharide of Streptococcus oralis C104: A 600-MHz NMR study

Journal Article · Tue Sep 03 00:00:00 EDT 1991 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00099a012· OSTI ID:5601602

Abeygunawardana, C; Bush, C A ^[1]; Cisar, J O ^[2]

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1. Univ. of Maryland, Baltimore (United States)
2. National Inst. of Dental Research, Bethesda, MD (United States)

Specific lectin-carbohydrate interactions between certain oral streptococci and actinomyces contribute to the microbial colonization of teeth. The receptor molecules of Streptococcus oralis, 34, ATCC 10557, and Streptococcus mitis J22 for the galactose and N-acetylgalactosamine reactive fimbrial lectins of Actinomyces viscosus and Actinomyces naeslundii are antigenically distinct polysaccharides, each formed by a different phosphodiester-linked oligosaccharide repeating unit. Receptor polysaccharide was isolated form S. oralis C104 cells and was shown to contain galactose, N-acetylgalactosamine, ribitol, and phosphate with molar ratios of 4:1:1:1. The {sup 1}H NMR spectrum of the polysaccharide shows that it contains a repeating structure. The individual sugars in the repeating unit were identified by {sup 1}H coupling constants observed in E-COSY and DQF-COSY spectra. NMR methods included complete resonance assignments ({sup 1}H and {sup 13}C) by various homonuclear and heteronuclear correlation experiments that utilize scalar couplings. Sequence and linkage assignments were obtained from the heteronuclear multiple-bond correlation (HMBC) spectrum. This analysis shows that the receptor polysaccharide of S. oralis C104 is a ribitol teichoic acid polymer composed of a linear hexasaccharide repeating unit containing two residues each of galactopyranose and galactofuranose and a residue each of GalNAc and ribitol joined end to end by phosphodiester linkages.

• OSTI ID: 5601602
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 30:35; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ACTINOMYCES
ALDEHYDES
AMINES
BACTERIA
BARYONS
CARBOHYDRATES
CARBON 13
CARBON ISOTOPES
DIGESTIVE SYSTEM
ELEMENTARY PARTICLES
EVEN-ODD NUCLEI
FERMIONS
GALACTOSE
GLUCOSAMINE
HADRONS
HEXOSAMINES
HEXOSES
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MEMBRANE PROTEINS
MICROORGANISMS
MOLECULAR STRUCTURE
MONOSACCHARIDES
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEONS
ORAL CAVITY
ORGANIC COMPOUNDS
POLYSACCHARIDES
PROTEINS
PROTONS
RECEPTORS
RESONANCE
SACCHARIDES
STABLE ISOTOPES
STREPTOCOCCUS
TEETH