(Genetics of the methanogenic bacterium Methanococcus voltae with attention to genetic control mechanisms): Progress report

Technical Report · Wed Jan 01 04:00:00 EST 1986

OSTI ID:6703730

The development of methanogen plasmid gene vectors would be greatly expedited by knowledge of the structure of methanogen transcription and translation signals. This would allow the directed insertion of a gene of choice downstream of the appropriate DNA sequences so as to bring about expression of that gene in the methanogen. The cloning of a Mc. voltae gene which complemented a hisA mutation in both E. coli and S. typhimurium has been reported. Characterization of the cloned gene indicated that both transcription and translation of the methanogen gene were initiated on the cloned methanogen DNA fragment. The methanogen hisA gene was sequenced and its sequence compared to a hisA-complementing gene isolated from Mc. vannielii. These studies demonstrated that each of these genes contained a eubacterial-like ribosome binding site (G-G-T-G) immediately upstream of the translation initiation codon which strongly suggests that methanogen messenger RNA can be recognized and translated by E. coli ribosomes and, perhaps, vice versa. 5 refs.

• Research Organization: Illinois Univ., Urbana (USA). Dept. of Microbiology
• DOE Contract Number: FG02-84ER13241
• OSTI ID: 6703730
• Report Number(s): DOE/ER/13241-1; ON: DE87007064
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
CLONING
DAYS LIVING RADIOISOTOPES
DNA SEQUENCING
DNA-CLONING
ENZYME INDUCTION
ENZYMES
EVEN-ODD NUCLEI
GENE REGULATION
GENE REPRESSORS
ISOMERASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MEMBRANE PORES
MEMBRANE TRANSPORT
MESSENGER-RNA
METHANOGENIC BACTERIA
MICROORGANISMS
MOLECULAR BIOLOGY
MUTAGENESIS
MUTANTS
NITROSO COMPOUNDS
NUCLEI
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLASMIDS
PROTEINS
RADIOISOTOPES
RNA
SPECIES DIVERSITY
STRUCTURAL CHEMICAL ANALYSIS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
TRANSCRIPTION