Structure and organization of the hisA gene of the Thermophilic archaebacterium Methanococcus thermolithotrophicus
Journal Article
·
· J. Bacteriol.; (United States)
OSTI ID:5381986
A restriction fragment of Methanococcus thermolithotrophicus genomic DNA was cloned into pUC8 to produce plasmid pET9301, which complements mutations in the hisA gene of Escherichia coli. Sequencing the DNA (2155 base pairs) cloned from this thermophilic methanogen demonstrated that the M. thermolithotrophicus hisA gene is located within a cluster of open reading frames (ORFs) and is 68 and 69% homologous at the nucleotide level to the hisA genes of the mesophilic methanococci M. voltae and M. vannielii, respectively. The ORF (ORF 206) immediately 5' to the hisA gene of M. thermolithotrophicus is partially deleted in the genomes of the two mesophilic species, whereas ORF 114, which is 5' to ORF 206, is conserved in all three species.
- Research Organization:
- Ohio State Univ., Columbus (USA)
- DOE Contract Number:
- AC02-81ER10945
- OSTI ID:
- 5381986
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 169:10; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Structure and sequence divergence of two archaebacterial genes
(Genetics of the methanogenic bacterium Methanococcus voltae with attention to genetic control mechanisms): Progress report
Structure and comparative analysis of the genes encoding component C of methyl coenzyme M reductase in the extremely thermophilic archaebacterium Methanothermus fervidus
Journal Article
·
Sat Jun 01 00:00:00 EDT 1985
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:6082587
(Genetics of the methanogenic bacterium Methanococcus voltae with attention to genetic control mechanisms): Progress report
Technical Report
·
Tue Dec 31 23:00:00 EST 1985
·
OSTI ID:6703730
Structure and comparative analysis of the genes encoding component C of methyl coenzyme M reductase in the extremely thermophilic archaebacterium Methanothermus fervidus
Journal Article
·
Sat Oct 01 00:00:00 EDT 1988
· Journal of Bacteriology; (USA)
·
OSTI ID:7067547