Structure and sequence divergence of two archaebacterial genes

Journal Article · Sat Jun 01 04:00:00 EDT 1985 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.82.12.4207· OSTI ID:6082587

Cue, D; Beckler, G S; Reeve, J N; Konisky, J

The DNA sequences of a region that includes the hisA gene of two related methanogenic archaebacteria, Methanococcus voltae and Methanococcus vannielii, have been compared. Both organisms show a similar genome organization in this region, displaying three open reading frames (ORFs) separated by regions of very high A+T content. Two of the ORFs, including ORFHisA, show significant DNA sequence homology. As might be expected for organisms having a genome that is A+T-rich, there is a high preference for A and U as the third base in codons. A ribosome binding site, G-G-T-G, is located 6 base pairs preceding the ATG translation initiation sequence of both hisA genes. The sequences upstream of the two hisA genes show only limited sequence homology. The M. voltae intergenic region contains four tandemly arranged repetitions of an 11-base-pair sequence, whereas the M. vannielii sequence contains both direct and inverted repetitive sequences. Based on the degree of hisA sequence homology, the authors conclude that M. voltae and M. vannielii are less closely related taxonomically than are members of the enteric group of eubacteria.

• Research Organization: Univ. of Illinois, Urbana
• DOE Contract Number: AC02-81ER10945; FG02-84ER13241
• OSTI ID: 6082587
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 82:12; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ADENINES
AMINES
ANTIMETABOLITES
AROMATICS
AZAARENES
AZINES
BACTERIA
BIOLOGICAL EVOLUTION
DNA SEQUENCING
DRUGS
GENES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
METHANOGENIC BACTERIA
MICROORGANISMS
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PURINES
PYRIMIDINES
STRUCTURAL CHEMICAL ANALYSIS
THYMINE
URACILS