Genetics of the methanogenic bacterium, Methanococcus voltae with attention to genetic expression mechanisms

Technical Report · Tue Jan 01 04:00:00 EST 1991

OSTI ID:5736781

Konisky, J

For this investigation of methanogen physiology and biochemistry we have studied Methanococcus voltae (M. voltae), a marine bacterium. We are currently analyzing two very important physiological systems: regulated expression of the membrane-associated P-type ATPase gene, and the molecular biology of coenzyme M (CoM) production. The RNA polymerase of M. voltae has been isolated and purified, and is being used to identify transcription factors and the promoter for the P-type ATPase gene. We have cloned and sequenced this gene, and have shown its expression is sodium dependent. We have isolated a CoM deficient mutant, and have cloned DNA which corrects the mutation. Parameters for electroporation of DNA into M. voltae have been determined. Additionally, we have characterized virus-like particles found in M. voltae, and determined that uptake of CoM from the environment may be characteristic of methanogens in an anaerobic marine environment. 5 refs., 1 fig.

• Research Organization: Illinois Univ., Urbana, IL (USA). Dept. of Microbiology
• Sponsoring Organization: DOE; USDOE, Washington, DC (USA)
• DOE Contract Number: FG02-84ER13241
• OSTI ID: 5736781
• Report Number(s): DOE/ER/13241-2; ON: DE91014937
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ALKANES
ATP-ASE
BACTERIA
BIOLOGICAL PATHWAYS
CHARGED PARTICLES
CLONING
COENZYMES
DNA HYBRIDIZATION
DNA-CLONING
DOCUMENT TYPES
ENZYME ACTIVITY
ENZYME INDUCTION
ENZYMES
GENE MUTATIONS
GENE REGULATION
GENES
GROWTH
HYBRIDIZATION
HYDROCARBONS
HYDROLASES
IONS
MEMBRANE PORES
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
METHANE
METHANOGENIC BACTERIA
MICROORGANISMS
MOLECULAR STRUCTURE
MUTAGENESIS
MUTATIONS
NUCLEOPROTEINS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHOHYDROLASES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PRODUCTION
PROGRESS REPORT
PROTEINS
PURIFICATION
RNA POLYMERASES
SODIUM IONS
TRANSCRIPTION FACTORS
TRANSFERASES
UPTAKE