Characterization of bromoethanesulfonate-resistant mutants of methanococcus voltae: evidence of a coenzyme M transport system

Journal Article · Sun Feb 01 04:00:00 EST 1987 · J. Bacteriol.; (United States)

OSTI ID:6470046

Santoro, N; Konisky, J

Mutants of Methanococcus voltae were isolated that were resistant to the coenzyme M (CoM; 2-mercaptoethanesulfonic acid) analog 2-bromoethanesulfonic acid (BES). The mutants displayed a reduced ability accumulate (/sup 35/S)BES relative to the sensitive parental strain. BES inhibited methane production from CH/sub 3/-S-CoM in cell extracts prepared from wild-type sensitive or resistant strains. BES uptake required the presence of both CO/sub 2/ and H/sub 2/ and was inhibited by N-ethylmaleimide and several reagents that are known to disrupt energy metabolism. The mutants showed normal uptake of isoleucine and were not cross-resistant to either azaserine or 5-methyltrypotophan and, thus, were neither defective in general energy-dependent substrate transport nor envelope permeability. Both HS-CoM and CH/sub 3/-S-CoM prevented the uptake of BES and protected cells from inhibition by it. The authors propose that M. voltae has an energy-dependent, carrier-mediated uptake system for HS-CoM and CH/sub 3/-S-CoM which can also mediate uptake of BES.

• Research Organization: Univ. of Illinois, Urbana
• DOE Contract Number: FG02-84ER13241
• OSTI ID: 6470046
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 169:2; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
550501 -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKANES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MARKERS
COENZYMES
DAYS LIVING RADIOISOTOPES
ESTERS
EVEN-ODD NUCLEI
GENETIC ENGINEERING
HYDROCARBONS
INHIBITION
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
METABOLISM
METHANE
METHANOGENIC BACTERIA
MICROORGANISMS
MUTANTS
NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIOISOTOPES
SULFONIC ACID ESTERS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
UPTAKE