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Genetic transformation in the methanogen Methanococcus voltae PS

Journal Article · · J. Bacteriol.; (United States)
OSTI ID:6405345
Mutations causing requirements for histidine, purine, and vitamin B/sub 12/ were obtained in strain PS of Methanococcus voltae (archaebacteria) upon irradiation with UV or gamma rays. The first two mutations were shown to revert at low frequencies and were sued to demonstrate the occurrence of transformation with homologous, wild-type DNA. The transformation rates obtained for these presumably chromosomal markers were in the range of 2 to 100 transformants per ..mu..g of DNA. Mutants resistant to 2-bromoethanesulfonate and to 5-methyl-DL-tryptophan were also isolated.
Research Organization:
California Institute of Technology, Pasadena
OSTI ID:
6405345
Journal Information:
J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 169:6; ISSN JOBAA
Country of Publication:
United States
Language:
English