Microbial ecology of bacterially mediated PCB biodegradation
Thesis/Dissertation
·
OSTI ID:6703079
The roles of plasmid mediated and consortia mediated polychlorinated biphenyl (PCB) biodegradation by bacterial populations isolated from PCB contaminated freshwater sediments were investigated. PCB degrading bacteria were isolated by DNA:DNA colony hybridization, batch enrichments, and chemostat enrichment. Analysis of substrate removal and metabolite production were done using chlorinated biphenyl spray plates, reverse phase high pressure liquid chromatography, Cl{sup {minus}} detection, and {sup 14}C-labeled substrate mineralization methods. A bacterial consortium, designated LPS10, involved in a concerted metabolic attack on chlorinated biphenyls, was shown to mineralize 4-chlorobiphenyl (4CB) and 4,4{prime}-dichlorobiphenyl (4,4{prime} CB). The LPS10 consortium was isolated by both batch and chemostat enrichment using 4CB and biphenyl (BP) as sole carbon source and was found to have tree bacterial isolates that predominated; these included: Pseudomonas, testosteroni LPS10A which mediated the breakdown of 4CB and 4,4{prime} CB to the putative meta-cleavage product and subsequently to 4-chlorobenzoic acid (4CBA), an isolate tentatively identified as an Arthrobacter sp. LPS10B which mediated 4CBA degradation, and Pseudomonas putida by A LPS10C whose role in the consortium has not been determined.
- Research Organization:
- Tennessee Univ., Knoxville, TN (USA)
- OSTI ID:
- 6703079
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BACTERIA
BIODEGRADATION
CARBON 14 COMPOUNDS
CHEMICAL REACTIONS
CHROMATOGRAPHY
CONTAMINATION
DECOMPOSITION
DNA HYBRIDIZATION
FRESH WATER
HYBRIDIZATION
HYDROCARBONS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MICROORGANISMS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLYCYCLIC AROMATIC HYDROCARBONS
PSEUDOMONAS
SEDIMENTS
SEPARATION PROCESSES
TRACER TECHNIQUES
WATER
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BACTERIA
BIODEGRADATION
CARBON 14 COMPOUNDS
CHEMICAL REACTIONS
CHROMATOGRAPHY
CONTAMINATION
DECOMPOSITION
DNA HYBRIDIZATION
FRESH WATER
HYBRIDIZATION
HYDROCARBONS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MICROORGANISMS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLYCYCLIC AROMATIC HYDROCARBONS
PSEUDOMONAS
SEDIMENTS
SEPARATION PROCESSES
TRACER TECHNIQUES
WATER