Bioavailability of a potato chromium complex to the laboratory rat

Thesis/Dissertation · Tue Jan 01 04:00:00 EST 1985

OSTI ID:6701738

Gilbert, H K

Research objectives were to study the effect of food source, preparation method and chemical form on bioavailability of chromium. Chromium concentration in potatoes was determined and tubers labeled either intrinsically or extrinsically with radioactive chromate. A labeled chromium complexes was isolated from preparations of raw, baked or fried potatoes and chromatographed on gel permeation media. Availability of the potato chromium complex to the rat was examined in three feeding studies. Animals were dosed with radioactive extrinsically or intrinsically labeled potato extract or with chromate. A labeled chromium complex was isolated from gastrointestinal contents of rats and chromatographed. Potato pulp and peel contained 1.63 and 2.70 ..mu..g Cr/g tissue respectively. True and apparent absorption from extrinsically labeled feedings were 33.4 +/- 4.7 and 29.8 +/- 11.2% respectively, and no differences existed between absorption from raw and cooked potatoes. Absorption from the extrinsic labeled potatoes differed significantly from absorption of inorganic chromatium. Apparent absorption of raw (11.1 +/- 7.9%) and cooked (-0.7 +/- 2.8%) intrinsically labeled feedings differed significantly. Absorption of inorganic chromium was 17.8% (true) and 11.5% (apparent). Examination of the chromium complex isolated from gastrointestinal tract contents showed enlargement of the complex in the stomach after consumption.

• Research Organization: Colorado State Univ., Fort Collins (USA)
• OSTI ID: 6701738
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL AVAILABILITY
CHROMATES
CHROMATOGRAPHY
CHROMIUM
CHROMIUM COMPLEXES
CHROMIUM COMPOUNDS
COMPLEXES
ELEMENTS
FOOD
GEL PERMEATION CHROMATOGRAPHY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
MAMMALS
METALS
OXYGEN COMPOUNDS
PLANTS
POTATOES
PURIFICATION
RATS
RODENTS
SEPARATION PROCESSES
TRACER TECHNIQUES
TRANSITION ELEMENT COMPLEXES
TRANSITION ELEMENT COMPOUNDS
TRANSITION ELEMENTS
TUBERS
VEGETABLES
VERTEBRATES