Neuropeptide K is present in human cerebrospinal fluid

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(90)90386-6· OSTI ID:6699324

Toresson, G; de las Carreras, C; Brodin, E; Bertilsson, L ^[1]

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1. Karolinska Institute, Stockholm (Sweden)

Neurokinin A-like immunoreactivity (NKA-LI) in human cerebrospinal fluid (CSF) was determined by radioimmuno assay (RIA) combined with high performance liquid chromatography (HPLC). The major immunoreactive component did not coelute with NKA, but coeluted with neuropeptide K (NPK), which contains the NKA sequence in its C-terminus. Trypsin treatment of this component from human CSF and of synthetic NPK, produced a substance which coeluted with NKA in the HPLC system. When the NKA-LI was oxidized with hydrogen peroxide and rechromatographed, the immunoreactivity coeluted with NPK sulfoxide. The results indicate that the main part of the NKA-LI in CSF is identical with NPK. The mean concentration of NPK measured in CSF from 6 healthy subjects by HPLC-RIA was 23 + 11 (SD) pmol/L.

• OSTI ID: 6699324
• Journal Information: Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 46:23; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
BIOASSAY
BIOLOGICAL MATERIALS
BODY FLUIDS
CEREBROSPINAL FLUID
CHROMATOGRAPHY
DIAGNOSTIC TECHNIQUES
ENZYMES
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
HYDROLASES
IMMUNE REACTIONS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
MATERIALS
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDE HYDROLASES
PEPTIDES
PEROXIDES
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
SEPARATION PROCESSES
SERINE PROTEINASES
TRACER TECHNIQUES
TRYPSIN
VERTEBRATES