Partial characterization of a novel endogenous opioid in human cerebrospinal fluid

Journal Article · Mon Dec 07 00:00:00 EST 1987 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(87)90438-3· OSTI ID:5243308

Miller, B E; Lipman, J J; Byrne, W L

Human cerebrospinal fluid (CSF) contains many uncharacterized endogenous opioids, in addition to the known enkephalins, endorphins, and dynorphins. These opioids may be separated by gel filtration chromatography and identified by radioreceptor assay for opioid activity. One region of the chromatographic elution profile, designated Peak B has previously been shown to be related to the pain status of chronic pain patients. The authors now report that human Peak B isolated from the CSF of pain-free elective surgery patients is present at a typical concentration equivalent in activity to 1.4 pmol of morphine sulfate per ml of CSF measured by radioreceptor assay. At a dose of 0.06 and 0.12 pmol morphine sulfate equivalents of CSF (MSE), injected into the cerebroventricular system of the mouse, Peak B produced an antinociceptive effect, the intensity and duration of which was dose-dependent and which was antagonized by naloxone. The mouse vas deferens (MVD) preparation was inhibited by Peak B in a manner that was sensitive to antagonism by naloxone only at low (< 1.0 ..mu..M) but not at higher (>6.0 ..mu..M) concentrations of the antagonist. Peak B activity in the MVD assay was unaffected by treatment with trypsin or ..cap alpha..-chymotrypsin. 32 references, 4 figures, 1 table.

• Research Organization: Univ. of Tennessee Center for Health Sciences, Memphis (USA)
• OSTI ID: 5243308
• Journal Information: Life Sci.; (United States), Vol. 41:23
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CEREBROSPINAL FLUID
CHEMICAL COMPOSITION
ENDORPHINS
RADIORECEPTOR ASSAY
CHROMATOGRAPHY
DOSE-RESPONSE RELATIONSHIPS
EXPERIMENTAL DATA
MICE
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL MATERIALS
BODY FLUIDS
DATA
DRUGS
INFORMATION
ISOTOPE APPLICATIONS
MAMMALS
MATERIALS
NEUROREGULATORS
NUMERICAL DATA
RODENTS
SEPARATION PROCESSES
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques