Radioreceptor assay of opioid peptides in selected canine brain regions
A radioreceptor assay using the opioid delta receptor-preferring ligand D-/sup 2/ala, D-/sup 5/leu leucine enkephalin (/sup 3/H-DADL) and the broader-specificity ligand /sup 3/H-etorphine was used to measure five HPLC-purified neuropeptide fractions derived from the peptide-rich fraction of tissue homogenates of nine anatomical regions of the canine brain. The receptoractive peptides studied were methionine enkephalin, alpha-neo-endorphin, dynorphin 1-8, methionine enkephalin-Arg-Phe, and leucine enkephalin. These peptides derive from two larger precursors: proenkephalin A, which contains methionine enkephalin, leucine enkephalin, methionine enkephalin-Arg-Phe; and proenkephalin B, which contains alpha-neo-endorphin and dynorphin 1-8. Receptoractive peptides were measured in the peptide-rich fraction derived from homogenates of canine hypothalamus, pituitary, caudate nucleus, amygdala, hippocampus, mid-brain, thalamus, pons-medulla, and cortex.
- Research Organization:
- Univ. of Tennessee, Memphis
- OSTI ID:
- 6171307
- Journal Information:
- Anal. Lett.; (United States), Journal Name: Anal. Lett.; (United States) Vol. 18:B15; ISSN ANALB
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
DATA
EXPERIMENTAL DATA
HYDROGEN ISOTOPES
INFORMATION
ISOTOPE APPLICATIONS
ISOTOPE EFFECTS
ISOTOPES
LIGHT NUCLEI
NERVOUS SYSTEM
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PROTEINS
RADIOISOTOPES
RADIORECEPTOR ASSAY
TRACER TECHNIQUES
TRITIUM
YEARS LIVING RADIOISOTOPES