Studies on a mutant regulatory protein synthesized by gene 45 of bacteriophage T4D: differential functional stabilization and suppression of temperature-sensitive characteristics

Technical Report · Sun Jan 01 04:00:00 EST 1978

OSTI ID:6693484

Baser, B R

Bacteriophage T4 diverts host cell metabolism from bacterial to viral functions after infection. The pattern is one of total, rapid, and irreversible host genome inactivation and degradation, and involves time-ordered sequences of gene expression generated by both transcriptional and posttranscriptional regulation. E. coli B infected with wild-type T4 at 37/sup 0/C synthesizes early proteins until about 12 min post-infection (mpi); this is called the S1 shutoff. When DNA synthesis is blocked by mutation, early protein synthesis continues to roughly 22 mpi; this shutoff (S2) is controlled by regA function. An understanding of the controls operating on and through bacteriophage P45 to regulate DNA synthesis and late transcription is therefore central to the understanding of late protein production and viral maturation.

• Research Organization: Rochester Univ., NY (USA). School of Medicine and Dentistry
• DOE Contract Number: EY-76-C-02-3490
• OSTI ID: 6693484
• Report Number(s): UR-3490/LCP-10
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BACTERIOPHAGES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
DNA
ESCHERICHIA COLI
GENETIC CONTROL
HOST-CELL REACTIVATION
KINETICS
MICROORGANISMS
MUTANTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PEST CONTROL
PROTEINS
REACTION KINETICS
RECOVERY
REPAIR
SYNTHESIS
TEMPERATURE DEPENDENCE
VIRUSES