Genetic mapping of regA mutants of bacteriophage T4D

Journal Article · Wed Jun 01 04:00:00 EDT 1977 · J. Virol.; (United States)

OSTI ID:7212901

Wiberg, J S; Mendelsohn, S L; Warner, V; Aldrich, C; Cardillo, T S

SP62, a mutant of bacteriophage T4 shown by Wiberg et al. (1973) to be defective in regulation of T4 protein synthesis, was shown by complementation tests to define a new gene, regA, and by intergenic mapping to lie between genes 43 and 62. The mapping involved crossing SP62 with a quadruple amber mutant defective in genes 42, 43, 62, and 44, selecting all six classes of amber-containing recombinants caused by single crossover events, and then scoring the presence or absence of SP62 in these recombinants. In addition, 15 new, spontaneous regA mutants were isolated, and 13 of these were mapped against each other; a total of eight different mutation sites were thus defined. Most of the new mutants were isolated as pseudorevertants of a leaky amber mutant in gene 62, according to Karam and Bowles (1974), whereas one was identified by virtue of the ''white ring'' around its plaque, a phenotype possessed by all the regA mutants at high temperature. SP62 was renamed regA1, and the new mutants were named regA2, regA3, etc.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 7212901
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 22:3; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIOPHAGES
BIOLOGY
GENE RECOMBINATION
GENETIC MAPPING
GENETICS
MICROORGANISMS
MUTANTS
PARASITES
VIRUSES