New late gene, dar, involved in the replication of bacteriophage T4 DNA. II. Overproduction of DNA binding protein (gene 32 protein) and further characterization

Journal Article · Sat Jul 01 04:00:00 EDT 1978 · J. Virol.; (United States)

OSTI ID:6677691

Wu, J R; Yen, Y C

We have previously shown that the arrested DNA synthesis of mutants defective in T4 phage gene 59 can be reversed by a mutation in dar. In this paper, we have examined the effect of the dar mutation on the kinetics of gene 32 protein (DNA binding protein) synthesis, DNA packaging, progeny formation, and several other processes. Several lines of evidence are presented showing that the regulation of synthesis of gene 32 protein is abnormal in dar1-infected cells. In these cells, gene 32 protein, an early protein, is also expressed late in the infectious cycle. Our data also indicate that the packaging of DNA into T4 phase heads is delayed in dar mutant-infected cells, and this in turn results in a 6- to 8-min delay in intracellular progeny formation, although the synthesis of late proteins appears to be normal, as shown by gel electrophoresis. We have also studied the phenotypes of the double mutant dar-amC5 (gene 59). The increased sensitivity to hydroxyurea caused by a mutation in the dar gene can be alleviated by a second mutation in gene 59, but an increased sensitivity to uv irradiation caused by a mutation in gene 59 cannot be alleviated by a second mutation in the dar gene. Therefore, the double mutant still exhibits abnormalities in the repair of uv lesions.

• Research Organization: Univ. of Arkansas, Little Rock
• OSTI ID: 6677691
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 27:1; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGICAL VARIABILITY
BIOSYNTHESIS
DNA
DNA REPLICATION
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GENE MUTATIONS
GENETIC VARIABILITY
IRRADIATION
MICROORGANISMS
MUTANTS
MUTATIONS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
SYNTHESIS
ULTRAVIOLET RADIATION
VIRUSES