Bacteriophage T4 gene 32 participates in excision repair as well as recombinational repair of UV damages

Journal Article · Sat Jun 01 00:00:00 EDT 1985 · Genetics; (United States)

OSTI ID:5176966

Mosig, G

Gene 32 of phage T4 has been shown previously to be involved in recombinational repair of UV damages but, based on a mutant study, was thought not to be required for excision repair. However, a comparison of UV-inactivation curves of several gene 32 mutants grown under conditions permissive for progeny production in wild-type or polA- hosts demonstrates that gene 32 participates in both kinds of repair. Different gene 32 mutations differentially inactivate these repair functions. Under conditions permissive for DNA replication and progeny production, all gene 32 mutants investigated here are partially defective in recombinational repair, whereas only two of them, P7 and P401, are also defective in excision repair. P401 is the only mutant whose final slope of the inactivation curve is significantly steeper than that of wild-type T4. These results are discussed in terms of interactions of gp32, a single-stranded DNA-binding protein, with DNA and with other proteins.

• Research Organization: Vanderbilt Univ., Nashville, TN
• OSTI ID: 5176966
• Journal Information: Genetics; (United States), Vol. 2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIOPHAGES
DNA REPAIR
ESCHERICHIA COLI
GENETIC RADIATION EFFECTS
DNA
DOSE-RESPONSE RELATIONSHIPS
GENE RECOMBINATION
GENES
MUTANTS
MUTATIONS
PROTEINS
ULTRAVIOLET RADIATION
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
VIRUSES
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)