Studies on the recombination genes of bacteriophage T4: suppression of uvsX and uvsY mutations by uvsW mutations

Journal Article · Sun Feb 01 04:00:00 EST 1987 · Genetics; (United States)

OSTI ID:6850754

Yonesaki, T; Minagawa, T

Genes uvsW, uvsX and uvsY are dispensable for T4 growth but are implicated in recombination and in the repair of damaged DNA. The authors found that large-plaque mutants arose efficiently from small-plaque uvsX and uvsY mutants at 42/sup 0/ and were pseudorevertants containing a new mutation in uvsW. Using reconstructed double mutants, they confirmed that a mutation in uvsW partially increases the burst size and UV resistance of uvsX and uvsY mutants. At 41/sup 0/ the uvsW mutation completely restores the arrest in DNA synthesis caused by mutations in genes uvsX, uvsY and 46, but at 30/sup 0/ it only partially restores DNA synthesis in a gene 46 mutant and does not restore DNA synthesis in uvsX and uvsY mutants. Restored DNA synthesis at 41/sup 0/ was paralleled by the overproduction of single-stranded DNA and gene 32 protein. Based on these findings, they propose that the uvsW gene regulates the production of single-stranded DNA and they discuss the phenotype of uvsW mutants and their suppression of some uvsX and uvsY phenotypes. Infection of restrictive cells with am uvsW mutants revealed a defect in the synthesis of a protein of molecular weight 53,000 daltons, suggesting that this protein is the uvsW gene product.

• Research Organization: Kyoto Univ., Japan
• OSTI ID: 6850754
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 115:2; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIOPHAGES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
DNA REPAIR
ELECTROMAGNETIC RADIATION
GENE RECOMBINATION
GENE REGULATION
GENES
MICROORGANISMS
MOLECULAR WEIGHT
MUTANTS
ORGANIC COMPOUNDS
PARASITES
PHENOTYPE
PROTEINS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
SYNTHESIS
ULTRAVIOLET RADIATION
VIRUSES