Enzymatic hydrolysis of corn stover process development and evaluation
Technical Report
·
OSTI ID:6668834
The hydrolysis of acid treated corn stover with cellulase from T. reesei Rut-C-30 was evaluated. Experiments were conducted with substrate concentrations of 5 to 25% by weight, enzyme activities of 0.5 to 7 IU/ml and residence times of 24 to 48 hours. Maximum conversion was 55% for specific cellulase activity of 25 to 30 IU/g. Optimum cellobiase activity for minimum cellobiose production was found to be approximately 1.8 cellobiase units to 1 FPA unit. Hydrolysis experiments with steam exploded corn stover led to a maximum conversion of 80%, significantly higher than the results obtained for acid treated substrate. Steam exploded corn stover was demonstrated as a suitable carbon source for growth of T. reesei in submerged cultures.
- Research Organization:
- Lawrence Berkeley Lab., CA (USA)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 6668834
- Report Number(s):
- LBL-14223; ON: DE83001379
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
ACIDIFICATION
AGRICULTURAL WASTES
CARBOHYDRATES
CELLOBIOSE
CELLULASE
CEREALS
CHEMICAL COMPOSITION
CHEMICAL REACTIONS
DECOMPOSITION
DISACCHARIDES
ENZYMATIC HYDROLYSIS
ENZYMES
FILTRATION
FUNGI
GLYCOSYL HYDROLASES
GRAMINEAE
GRASS
HYDROLASES
HYDROLYSIS
LYSIS
MACHINING
MAIZE
MILLING
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC WASTES
PLANTS
SACCHARIDES
SEPARATION PROCESSES
SOLVOLYSIS
STEAM
TRICHODERMA
TRICHODERMA VIRIDE
WASTES
140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
ACIDIFICATION
AGRICULTURAL WASTES
CARBOHYDRATES
CELLOBIOSE
CELLULASE
CEREALS
CHEMICAL COMPOSITION
CHEMICAL REACTIONS
DECOMPOSITION
DISACCHARIDES
ENZYMATIC HYDROLYSIS
ENZYMES
FILTRATION
FUNGI
GLYCOSYL HYDROLASES
GRAMINEAE
GRASS
HYDROLASES
HYDROLYSIS
LYSIS
MACHINING
MAIZE
MILLING
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC WASTES
PLANTS
SACCHARIDES
SEPARATION PROCESSES
SOLVOLYSIS
STEAM
TRICHODERMA
TRICHODERMA VIRIDE
WASTES