Kinetics of cellobiose hydrolysis using cellobiase composites from Trichoderma reesei and Aspergillus niger
The enzymatic hydrolysis of cellulose to glucose involves the formation of cellobiose as an intermediate. It has been found necessary to add cellobiase from Aspergillus niger (NOVO) to the cellobiase component of Trichoderma reesei mutant Rut C-30 (Natick) cellulase enzymes in order to obtain after 48 h complete conversion of the cellobiose formed in the enzymatic hydrolysis of biomass. This study of the cellobiase activity of these two enzyme sources was undertaken as a first step in the formation of a kinetic model for cellulose hydrolysis that can be used in process design. In order to cover the full range of cellobiose concentrations, it was necessary to develop separate kinetic parameters for high- and low-concentration ranges of cellobiose for the enzymes from each organism. Competitive glucose inhibition was observed with the enzymes from both organisms. Substrate inhibition was observed only with the A. niger enzymes.
- Research Organization:
- Dartmouth College, Hanover, NH
- OSTI ID:
- 5659294
- Journal Information:
- Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 27:4; ISSN BIBIA
- Country of Publication:
- United States
- Language:
- English
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BIOCHEMICAL REACTION KINETICS
CARBOHYDRATES
CELLOBIOSE
CELLULOSE
CHEMICAL REACTIONS
DATA
DECOMPOSITION
DISACCHARIDES
DISPERSIONS
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
INFORMATION
KINETICS
LYSIS
MATHEMATICAL MODELS
MIXTURES
NUMERICAL DATA
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
REACTION KINETICS
SACCHARIDES
SOLVOLYSIS