Hypoxic fraction determinations with the BA1112 rat sarcoma: variation within and among assay techniques
Journal Article
·
· Radiat. Res.; (United States)
Measurement of the hypoxic fraction in the same tumor system by different assay techniques often gives incompatible results. The hypoxic fraction of the BA1112 sarcoma has been measured by three assay techniques: tumor control and growth delay assays in which the tumors remain in situ after irradiation, and paired survival curve assays in which the tumors are excised after irradiation. The assays were done with and without anesthesia on tumors growing in two different subcutaneous sites. Interpretation of the results was complicated by disagreements between the growth delay and tumor control assays, and by disparities between replicate determinations. The discrepancies among assays are caused almost entirely by variations in the response of the artifically hypoxic tumors, rather than variations in the response of aerobic tumors. These results indicate that assumptions commonly made when measuring hypoxic fractions may not be valid.
- Research Organization:
- Medical Coll. of Wisconsin, Milwaukee
- OSTI ID:
- 6667299
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 98:3; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANESTHESIA
ANIMAL CELLS
ANIMALS
ANOXIA
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DISEASES
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
IONIZING RADIATIONS
IRRADIATION
MAMMALS
NEOPLASMS
RADIATION EFFECTS
RADIATIONS
RATS
RODENTS
SARCOMAS
TUMOR CELLS
VERTEBRATES
WHOLE-BODY IRRADIATION
X RADIATION
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANESTHESIA
ANIMAL CELLS
ANIMALS
ANOXIA
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DISEASES
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
IONIZING RADIATIONS
IRRADIATION
MAMMALS
NEOPLASMS
RADIATION EFFECTS
RADIATIONS
RATS
RODENTS
SARCOMAS
TUMOR CELLS
VERTEBRATES
WHOLE-BODY IRRADIATION
X RADIATION