Mass spectral investigations on toxins. 9. Application of liquid chromatography/mass spectrometry techniques for the detection and quantification of macrocyclic trichothecenes in Brazilian plant samples. Technical report, March-July 1985
Trichothecenes are toxic, fungal metabolites that have been associated with human health hazards, farm-product, livestock, and other animal losses for decades. Allegedly, these metabolites were also used as chemical warfare agents. Recently, the growth of trichothecene-producing fungi was detected also in U.S. urban environments. Thus, the development of rapid, specific, sensitive, and accurate methods for analyzing these toxins in environmental, agricultural, and biological samples has become vital. The large number of trichothecenes present and their polar and labile properties pose a great challenge in developing a common methodology to analyze all the derivatives of interest. Thermally labile, polar, toxic roridins and biologically active baccharinoids were separated over a reverse phase, high-performance liquid-chromatographic column and effectively ionized under thermospray ionization conditions. The mass spectra indicated the formation of corresponding molecular ion-ammonium adducts in great abundance. Experiments designed for monitoring specific ions of these analytes at predesignated intervals were used to accurately analyze these macrocyclic trichothecenes in real, crude samples. A synthetically modified macrocyclic trichothecene, 8-ketoverrucarin A, was used as the internal standard for detecting and quantifying these compounds. The minimum detectable limits were from 1 to 2 nanograms.
- Research Organization:
- Chemical Research, Development and Engineering Center, Aberdeen Proving Ground, MD (USA)
- OSTI ID:
- 6661775
- Report Number(s):
- AD-A-197289/2/XAB; CRDEC-TR-88074
- Country of Publication:
- United States
- Language:
- English
Similar Records
Protection against trichothecene mycotoxins
Metabolic products, mass spectral analyses, and synthesis of toxic trichothecenes. Final report, 15 April 1982-14 July 1985
Related Subjects
400102* -- Chemical & Spectral Procedures
ANTIGENS
CHEMICAL ANALYSIS
CHEMICAL WARFARE AGENTS
CHROMATOGRAPHY
DETECTION
DOCUMENT TYPES
HAZARDOUS MATERIALS
HAZARDS
MASS SPECTRA
MATERIALS
METABOLITES
MONITORING
PROGRESS REPORT
SEPARATION PROCESSES
SPECTRA
TOXIC MATERIALS
TOXICITY
TOXINS
URBAN AREAS
WEAPONS