Mass-spectral investigations on toxins. 3. Accurate analysis and quantitation of macrocyclic trichothecenes in environmental, fungal, fermentation, and Brazilian plant samples by gas-chromatographic/negative ion chemical ionization-mass spectrometric techniques. Technical report, January 1983-October 1984
A general, sensitive Gas Chromatographic Negative Ion Chemical Ionization-Mass Spectrometric method of analysis was developed for accurately detecting and measuring several polar, thermally labile,toxic macrocyclic trichothecenes. The procedure involves the conversion of the molecules into their corresponding alcohols (Verrucarols) by alkaline hydrolysis, followed by the derivatization of the hydrolysates with heptafluorobutyrylimidazole and analysis by the gas chromatographic/mass spectrometric techniques under negative ion chemical inoization conditions. Using this procedure, trace quantities (0.25-2 micrograms) of several macrocyclic trichothecenes with different verrucarol and ester moieties were analyzed successfully with good precision. The method developed was applicable for the accurate analysis of at least low part-per-billion levels of these macrocyclic trichothecenes in real-life samples such as fungal products, fermentation broths, and plant samples. This is the first report describing the well-developed, sensitive, and applicable method for detecting and measuring these compounds in true samples.
- Research Organization:
- Chemical Research, Development and Engineering Center, Aberdeen Proving Ground, MD (USA)
- OSTI ID:
- 6292015
- Report Number(s):
- AD-A-180963/1/XAB; CRDEC-TR-87011
- Country of Publication:
- United States
- Language:
- English
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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400201* -- Chemical & Physicochemical Properties
98 NUCLEAR DISARMAMENT, SAFEGUARDS, AND PHYSICAL PROTECTION
ANIONS
ANTIGENS
BIOCONVERSION
CHARGED PARTICLES
CHEMICAL REACTIONS
CHEMICAL WARFARE AGENTS
CHROMATOGRAPHY
CONVERSION
DECOMPOSITION
DETECTION
ESTERS
FERMENTATION
FLUIDS
FUNGI
GAS CHROMATOGRAPHY
GASES
HYDROLYSIS
IONIZATION
IONS
LYSIS
MASS SPECTRA
MATERIALS
MOLECULES
ORGANIC COMPOUNDS
PLANTS
SAMPLING
SENSITIVITY
SEPARATION PROCESSES
SOLVOLYSIS
SPECTRA
TOXIC MATERIALS
TOXINS
WEAPONS