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A kinetic study of the in vivo incorporation of /sup 65/Zn into the rat hippocampus

Journal Article · · J. Neurosci.; (United States)
OSTI ID:6660794
; ;
Previous autoradiographical studies utilizing /sup 65/Zn demonstrated an apparent concentration of /sup 65/Zn in the mossy fiber boutons of the hippocampus. To examine the speciation of the /sup 65/Zn pool found in this neuronal pathway, we investigated the in vivo incorporation of systemic /sup 65/Zn into rat hippocampus compared with other brain regions. We were especially interested in kinetically assessing the zinc associated with three previously identified cytosolic zinc-binding species found in the hippocampus. The hypothesis that two of these cytosolic zinc-binding species, a metallothionein-like protein and a putative zinc-glutathione complex, may be responsible for the sequestration of zinc in the hippocampus was tested. It was confirmed that the t 1/2 of hippocampal zinc is longer than other brain regions that were studied. Furthermore, we observed that /sup 65/Zn is incorporated into three cytosolic zinc-binding species in the hippocampus as resolved using Ultrogel AcA 34 gel permeation chromatography. One of these species, the putative zinc-glutathione complex, accumulates zinc more slowly than the other species. The data suggest that the putative zinc-glutathione complex may represent an important /sup 65/Zn pool in the hippocampus. This finding is in accordance with out hypothesis that a zinc-binding species, specifically, the putative zinc-glutathione complex, may be responsible for the sequestration of zinc in the hippocampal mossy boutons.
Research Organization:
Johns Hopkins University, School of Hygiene and Public Health, Department of Environmental Health Sciences, Baltimore, Maryland
OSTI ID:
6660794
Journal Information:
J. Neurosci.; (United States), Journal Name: J. Neurosci.; (United States) Vol. 4:6; ISSN JNRSD
Country of Publication:
United States
Language:
English

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Related Subjects

560172* -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHEMICAL BONDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPES
MAMMALS
NERVOUS SYSTEM
NUCLEI
ORGANS
RADIOISOTOPES
RADIONUCLIDE KINETICS
RATS
RODENTS
UPTAKE
VERTEBRATES
ZINC 65
ZINC ISOTOPES