Radioisotopic investigations of zinc uptake into brain slices

Thesis/Dissertation · Sat Jan 01 04:00:00 EST 1983

OSTI ID:5808180

Howell, G A

The presence of zinc in the vicinity of the hippocampal mossy fibers has been repeatedly demonstrated, and several lines of evidence suggest that the mossy-fiber zinc is concentrated within the terminals of mossy fibers. In search of insight into the metabolism and function of mossy-fiber zinc, the present study investigated the transport of zinc into tissue slices and the response of the zinc transport to depolarization. Kinetic analysis of zinc accumulation by mouse brain slices in vitro revealed the presence of a high affinity uptake component with an apparent Km of 17.7 ..mu..M for hippocampus, 16.6 ..mu..M< for cortex and 25 ..mu..M for striatum and a V/sub max/ of 9.2 ng/mg/hr for the hippocampus, 10.1 ng/mg/hr for cortex and 9.6 ng/mg/hr for striatum. Cytoarchitectonic differences in zinc transport between the different hippocampal subregions were found with those regions containing granule cells or mossy fiber axons accumulating greater amounts of zinc than the CA 1 region. The present finding that mossy-fiber neuropil selectivity accumulates zinc suggests the presence of a zinc-binding substance unique to mossy-fiber tissue.

• Research Organization: Texas Univ., Dallas (USA)
• OSTI ID: 5808180
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
560305 -- Chemicals Metabolism &amp; Toxicology-- Vertebrates-- (-1987)
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
DISTRIBUTION
ELEMENTS
ISOTOPES
KINETICS
MAMMALS
METALS
MICE
NERVOUS SYSTEM
ORGANS
RODENTS
TISSUE DISTRIBUTION
UPTAKE
VERTEBRATES
ZINC
ZINC ISOTOPES