The chemorepellent draxin is involved in hippocampal mossy fiber projection
- Department of Developmental Neurobiology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto 860-8556 (Japan)
- Department of Project Programs, Institute of Development, Aging and Cancer (IDAC), Tohoku University, Sendai 980-8575 (Japan)
Highlights: • Draxin deficiency led to abnormal projection of hippocampal mossy fibers. • Draxin receptors were expressed on granule cells dissociated from the dentate gyrus. • Draxin inhibited the growth of hippocampal mossy fibers in vitro. Lamina-specific afferent innervation of the mammalian hippocampus is critical for its function. We investigated the relevance of the chemorepellent draxin to the laminar projections of three principal hippocampal afferents: mossy fibers, entorhinal, and associational/commissural fibers. We observed that draxin deficiency led to abnormal projection of mossy fibers but not other afferents. Immunohistochemical analysis indicated that draxin is expressed in the dentate gyrus and cornu ammonis (CA) 3 at postnatal day 0, when dentate granule cells begin to extend mossy fibers towards CA3. Furthermore, a neurite growth assay using dissociated cells of the neonatal dentate gyrus revealed that draxin inhibited the growth of calbindin-D28k-expressing mossy fibers in vitro. Taken together, we conclude that draxin is a key molecule in the regulation of mossy fiber projections.
- OSTI ID:
- 23137122
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 500, Issue 2; Other Information: Copyright (c) 2018 Elsevier Inc. All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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