Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Cytosolic distribution of trace metals and chlorinated hydrocarbon oxygenated metabolistes in fish from coastal southern California

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5321631
Liver and gonad cytosols from four fish species collected from municipal outfall and reference sites were separated by Sephadex G-75 column chromatography into pools containing enzymes (ENZ), metallothionein (MT) and glutathione (GSH). Cadmium (Cd), copper (Cu) and zinc (Zn) were usually lower in fish livers from near the outfall. Most Cd, Cu, and Zn occurred in MT pools of livers and gonads. However, Zn and Cu occurred predominately in the ENZ pools at the lowest observed concentrations. In all species, ENZ-Cu showed positive slopes when regressed against total-cytosolic-Cu. ENZ-Cd seldom showed positive slopes when regressed against total-cytosolic-Cd. Patterns for Zn were the least consistent among species with highest ENZ-Zn slopes in livers with the lowest cytosolic-Zn concentrations. The largest portion of oxygenated-DDT and -PCB metabolites (MTBs) was partitioned into liver GSH pools. Concentrations of MTBs in ENZ pools showed positive slopes when regressed against total-cytosolic- and GSH-MTBs. Results for Cu and MTBs are consistent with the notion of an equilibrium-dependent exchange of these between cytosolic pools with no threshold for toxicity.
Research Organization:
Southern California Coastal Water Research Project, Long Beach
OSTI ID:
5321631
Report Number(s):
CONF-8604222-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
Country of Publication:
United States
Language:
English