Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Histological comparisons of white pine needles fumigated with ozone and sulfur dioxide singly and in mixtures

Journal Article · · Proc. Pa. Acad. Sci.; (United States)
OSTI ID:6656523
Needles of Pinus strobus were fumigated with low concentrations of ozone and sulfur dioxide singly and in mixtures for time periods of less than one hour. The needles were killed and fixed immediately after fumigation, cross section 15 microns thick were cut and then stained in a Safranin O-Fast Green stain series. The data revealed that ozone appeared to have a greater initial effect than sulfur dioxide on the tissues of the needles. The sections cut from needles fumigated with sulfur dioxide were similar in appearance to the tissues from nonfumigated control needles. The injury observed in sections of needles fumigated with a mixture of ozone and sulfur dioxide was characteristic of injury caused by ozone rather than sulfur dioxide. Data indicated that fumigations by low concentrations of ozone for a time period of less than one hour could cause changes in the tissues that would be injurious to the normal activities of the needles.
Research Organization:
Susquehanna Univ., Selingsgrove, PA
OSTI ID:
6656523
Journal Information:
Proc. Pa. Acad. Sci.; (United States), Journal Name: Proc. Pa. Acad. Sci.; (United States) Vol. 54; ISSN PPASA
Country of Publication:
United States
Language:
English