Determination of vitamin B/sub 6/ bioavailability in animal tissues using intrinsic and extrinsic labeling in the rat

Journal Article · · J. Agric. Food Chem.; (United States)

OSTI ID:6654624

Ink, S L; Gregory, III, J F; Sartain, D B

The effect of thermal processing on the bioavailability of vitamin B/sub 6/ in liver and muscle was examined by radioisotopic enrichment of these tissues. Rats were fed a single gelled test meal containing rat liver or muscle intrinsically enriched by vascular perfusion with (/sup 3/H)vitamin B/sub 6/ or a gelled test meal containing (/sup 3/H)pyridoxine (PN). Diets were extrinsically enriched with (/sup 14/C)PN to permit a direct comparison of enrichment methods. Absorption and metabolism were examined by analysis of tissues and excreta 24 h after the test meal had been consumed. The bioavailability of (/sup 3/H)B/sub 6/ vitamers in the raw tissues was equivalent to that of (/sup 3/H)PN in controls. Thermal processing of the tissues (121/sup 0/C, 45 min) induced destruction of 25-30% of the (/sup 3/H)B/sub 6/ vitamers and weakly reduced (less than or equal to10%) the utilization of the remaining(/sup 3/H)B/sub 6/ vitamers. The presence of monosodium glutamate (MSG) during thermal processing did not alter the results. The utilization of (/sup 14/C)PN was unaffected by diet composition. These data demonstrate the high bioavailability of vitamin B/sub 6/ in animal-derived foods and support the use of isotopic enrichment methods as an alternative to conventional bioassay procedures.

• Research Organization: Univ. of Florida, Gainesville
• OSTI ID: 6654624
• Journal Information: J. Agric. Food Chem.; (United States), Vol. 34:6
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
VITAMIN B GROUP
BIOLOGICAL AVAILABILITY
METABOLISM
CARBON 14 COMPOUNDS
DIET
LIVER
MUSCLES
PYRIDOXINE
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
AZINES
BODY
DIGESTIVE SYSTEM
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIDINES
RODENTS
VERTEBRATES
VITAMINS
550501* - Metabolism- Tracer Techniques