Fate of dietary pyridoxine-beta-glucoside in the lactating rat
(/sup 3/H)5'-O-(beta-D-glucopyranosyl) pyridoxine (PN-glucoside) and (/sup 14/C)pyridoxine (PN) were orally administered to lactating rats. Milk was collected from the dam, and the stomach contents and liver were collected from the suckling pups 24 and 48 h after administration. Analysis of the isotopic ratio (/sup 3/H//sup 14/C) in the milk and stomach contents indicated that the secretion of /sup 3/H in the milk was 20-25% as great as the secretion of /sup 14/C. The only labeled form of /sup 3/H and /sup 14/C in the stomach contents was pyridoxal phosphate (PLP), indicating that PN-glucoside was hydrolyzed to PN and subsequently metabolized prior to secretion by the mammary gland. The isotopic ratio in the livers of the pups was similar to that of the stomach contents. Furthermore, the relative distribution of the two isotopes among the hepatic metabolites of the pups was similar. The results of this study indicate that intact PN-glucoside is not secreted in milk, although vitamin B-6 derived from the limited hydrolysis and metabolism of PN-glucoside is delivered to the mammary gland for secretion.
- Research Organization:
- Univ. of Florida, Gainesville (USA)
- OSTI ID:
- 6270906
- Journal Information:
- J. Nutr.; (United States), Journal Name: J. Nutr.; (United States) Vol. 119:1; ISSN JONUA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AZINES
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CARBOHYDRATES
CARBON 14 COMPOUNDS
COUNTING TECHNIQUES
DIET
DIGESTIVE SYSTEM
FOOD
GASTROINTESTINAL TRACT
GLANDS
GLYCOSIDES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPE RATIO
LABELLED COMPOUNDS
LACTATION
LIVER
MAMMALS
MATERIALS
METABOLISM
MILK
ORAL ADMINISTRATION
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PREGNANCY
PYRIDINES
PYRIDOXINE
RATS
RODENTS
SCINTILLATION COUNTING
STOMACH
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
VITAMIN B GROUP
VITAMINS