Human bradykinin B2 receptor: Nucleotide sequence analysis and assignment to chromosome 14
- ICI Pharmaceuticals, Cheshire (United Kingdom)
Functional cDNA clones for human bradykinin B2 receptor were isolated from uterus RNA by a polymerase chain reaction (PCR)-based method and by screening a human cosmid library with rat bradykinin B2 receptor probe. We isolated several overlapping clones from the cosmid library, each of which encodes the entire protein-coding sequence. The human bradykinin B2 receptor gene codes for a 364-amino-acid protein with a molecular mass of 41,442 Da that is highly homologous to rat bradykinin B2 receptor cDNA (81%). The entire human cDNA sequence was cloned into an expression vector and mRNA was synthesised by in vitro transcription. Applications of bradykinin caused membrane current responses in Xenopus oocytes injected with the in vitro-synthesized mRNA. Preincubation with the potent B2 antagonist, HOE140, prevented this response. The genomic clone is intronless, and we have identified an upstream promoter region and a downstream polyadenylation signal. The human bradykinin B2 receptor gene has been mapped to chromosome 14 using PCR to specifically amplify DNA from somatic cell hybrids. 10 refs., 1 fig., 1 tab.
- OSTI ID:
- 6652873
- Journal Information:
- Genomics; (United States), Vol. 15:2; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
Similar Records
Molecular cloning, chromosomal mapping, and functional expression of human brain glutamate receptors
Mapping of the human bradikinin B2 receptor gene and GALC gene at 14q31-32.1, the region of the Machado Joseph disease locus
Related Subjects
BRADYKININ
RECEPTORS
HUMAN CHROMOSOME 14
GENETIC MAPPING
DNA SEQUENCING
COSMIDS
DNA
DNA-CLONING
GENE AMPLIFICATION
LIBRARIES
OOCYTES
RNA
SCREENING
TRANSCRIPTION
UTERUS
BODY
CHROMOSOMES
CLONING
DNA HYBRIDIZATION
FEMALE GENITALS
GERM CELLS
HUMAN CHROMOSOMES
HYBRIDIZATION
KININS
MAPPING
MEMBRANE PROTEINS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics