Identification of the transcriptional unit, structural organization, and promoter sequence of the human sex-determining region Y (SRY) gene, using a reverse genetic approach

Journal Article · Fri Jan 01 04:00:00 EST 1993 · American Journal of Human Genetics; (United States)

OSTI ID:6616131

Su, Hua; Lau, Y F.C. ^[1]

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1. Univ. of California, San Francisco (United States)

Using a simple strategy involving cosmid-mediated gene transfer, cDNA library construction, and molecular characterization techniques, the authors have determined the transcriptional unit, structural organization, and promoter sequence of the human sex-determining region Y (SRY) gene, the putative testis-determining factor (TDF) gene on the human Y chromosome. By this approach, a recombinant cosmid harboring the human SRY sequence was isolated and transfected to appropriate tissue-cultured cells. Recombinant cDNA clones were isolated from a cDNA library constructed from poly (A) + RNA of the transfected cells. Comparative studies between the respective cDNAs and the genomic cosmid have provided information regarding the organization of the SRY gene and its mRNAs. The results indicate that the human SRY gene is an intronless gene, produces transcripts of 1.1 kb, and possesses promoter activities in the transfected cells at approximately 310 bp of its upstream sequences. 57 refs., 5 figs.

• OSTI ID: 6616131
• Journal Information: American Journal of Human Genetics; (United States), Journal Name: American Journal of Human Genetics; (United States) Vol. 52:1; ISSN AJHGAG; ISSN 0002-9297
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMES
CLONING
CONSTRUCTION
COSMIDS
DNA HYBRIDIZATION
DNA-CLONING
GENE REPRESSORS
GENES
HETEROCHROMOSOMES
HUMAN CHROMOSOMES
HUMAN Y CHROMOSOME
HYBRIDIZATION
LIBRARIES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RNA
SEX
Y CHROMOSOME