Cloning and characterization of SOX5, a new member of the human SOX gene family
- Univ. of Cambridge (United Kingdom)
- Institute of Cancer Research, London (United Kingdom)
The mammalian Y-linked testis determining gene, SRY, encodes a protein with a DNA binding motif known as the HMG box. A large family of genes sharing a high similarity with the SRY HMG box and named Sox (Sry-related HMG box) in mouse and SOX in human has been identified from various organisms. We have cloned SOX5, a new member of the human SOX gene family. SOX5 cDNAs isolated from a human adult testis cDNA library show a high similarity with the mouse Sox5 transcript over a large region identical in all the human cDNAs. However, comparison of the 5{prime} unique sequences of the cDNAs suggests that the SOX5 gene is subject to alternative splicing. Genomic analysis identified a SOX5 pseudogene located on 8q21.1, whereas the SOX5 gene itself, which contains a minimum of five introns, maps to 12p12.1. In contrast to the mouse gene, the human SOX5 gene is expressed in a variety of human tissues, and different size transcripts are observed in adult testis and fetal brain. 19 refs., 5 figs.
- OSTI ID:
- 486324
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 36; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
AMINO ACID SEQUENCE
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
FLUORESCENCE
GENE REGULATION
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 12
HUMAN CHROMOSOME 8
HUMAN Y CHROMOSOME
NUCLEOTIDES
POLYMERASE CHAIN REACTION
SPLICING
STRUCTURE-ACTIVITY RELATIONSHIPS
TESTES
TISSUE DISTRIBUTION
TRANSCRIPTION