Genomic DNA sequence, expression, and chromosomal localization of the human B1 bradykinin receptor gene BDKRB1

Journal Article · Mon Jan 01 04:00:00 EST 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0008· OSTI ID:478833

Chai, K X; Chao, J; Chao, L ^[1]

---

1. Medical Univ. of South Carolina, Charleston, SC (United States); and others

We have cloned and sequenced the human B1 bradykinin receptor gene (BDKRB1), which contains as uninterrupted coding exon. A putative promoter was identified by linking various lengths of the 5{prime}-flanking region of the B1 receptor gene coding sequence to a CAT reporter and assaying for CAT activity. Deletion analysis showed that a 300-bp fragment in the promoter region is sufficient to direct the synthesis of the reporter and that an enhancer-like element is present between -1842 and -812. A genomic Southern blot using the B1 cDNA revealed that the receptor is encoded by a single-copy gene. The gene is located on chromosome 14q32.1-32.2, in close proximity to the B2 receptor gene. Northern blot analysis identified a 1.7- to 1.8-kb mature mRNA transcript of the B1 receptor gene in the kidney and pancreas. A widespread tissue distribution of the B1 gene expression was identified by RT-PCR-Southern blot analysis using specific oligonucleotide probes. 25 refs., 6 figs.

• OSTI ID: 478833
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 31; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOASSAY
BRADYKININ
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GENE REGULATION
GENES
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOME 14
OLIGONUCLEOTIDES
PATIENTS
POLYMERASE CHAIN REACTION
PROBES
RECEPTORS
STRUCTURE-ACTIVITY RELATIONSHIPS
TISSUE DISTRIBUTION
TRANSCRIPTION