Effects of benzene inhalation on murine pluripotent stem cells

Journal Article · Fri Jan 01 04:00:00 EST 1982 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398209530174· OSTI ID:6652361

Cronkite, E P; Inoue, T; Carsten, A L; Miller, M E; Bullis, J E; Drew, R T

Effects of benzene inhalation on mouse pluripotent hematopoietic stem cells have been evaluated. Male mice 8 to 12 wk old were exposed to 400 ppM benzene for 6 h/d, 5 d/wk, for up to 9 1/2 wk. At various time intervals exposed and control animals were killed, and cardiac blood was evaluated for changes in white blood cell (WBC) and red blood cell (RBC) content. In addition, femora and tibiae were evaluated for total marrow cellularity, stem cell content (as measured by the spleen colony technique), and the percent of stem cells in DNA synthesis (as determined by the tritiated thymidine cytocide technique). Exogenous spleen colonies grown from marrow of exposed animals were counted, identified, and scored by histological type. Exposure to benzene caused significant depressions of RBCs and WBCs throughout the exposure period, which continued for at least 14 d after exposure. Bone marrow cellularity and stem cell content were also depressed in exposed animals throughout the study. Tritiated thymidine cytocide of spleen colon-forming cells was generally increased in exposed animals, perhaps indicating a compensatory response to the reduction of circulating cells. Spleen colonies of all types were depressed after exposure to benzene. The significance of the reduction in cellularity, stem cell content, and changes in morphology of spleen colonies is discussed in relation to cellular toxicity and residual injury.

• Research Organization: Brookhaven National Lab., Upton, NY
• DOE Contract Number: AC02-76CH00016
• OSTI ID: 6652361
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 9; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AROMATICS
AZINES
BENZENE
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BLOOD COUNT
BODY
BODY FLUIDS
BONE MARROW
COLONY FORMATION
DNA
ERYTHROCYTES
HEMATOPOIETIC SYSTEM
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
INHALATION
INTAKE
LABELLED COMPOUNDS
LEUKOCYTES
MALES
MAMMALS
MATERIALS
MICE
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RIBOSIDES
RODENTS
SOMATIC CELLS
SPLEEN COLONY FORMATION
STEM CELLS
SYNTHESIS
THYMIDINE
TISSUES
TRITIUM COMPOUNDS
VERTEBRATES