Identification of human rotavirus serotype by hybridization to polymerase chain reaction-generated probes derived from a hyperdivergent region of the gene encoding outer capsid protein VP7

Journal Article · Wed Aug 01 00:00:00 EDT 1990 · Journal of Virology; (USA)

OSTI ID:6644047

Flores, J; Sears, J; Schael, I P; White, L; Garcia, D; Lanata, C; Kapikian, A Z ^[1]

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1. National Institutes of Health, Bethesda, MD (USA)

We have synthesized {sup 32}P-labeled hybridization probes from a hyperdivergent region (nucleotides 51 to 392) of the rotavirus gene encoding the VP7 glycoprotein by using the polymerase chain reaction method. Both RNA (after an initial reverse transcription step) and cloned cDNA from human rotavirus serotypes 1 through 4 could be used as templates to amplify this region. High-stringency hybridization of each of the four probes to rotavirus RNAs dotted on nylon membranes allowed the specific detection of corresponding sequences and thus permitted identification of the serotype of the strains dotted. The procedure was useful when applied to rotaviruses isolated from field studies.

• OSTI ID: 6644047
• Journal Information: Journal of Virology; (USA), Journal Name: Journal of Virology; (USA) Vol. 64:8; ISSN JOVIA; ISSN 0022-538X
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
DAYS LIVING RADIOISOTOPES
DNA
DNA HYBRIDIZATION
ENZYMES
FECES
GENES
GLYCOPROTEINS
HYBRIDIZATION
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MATERIALS
MICROORGANISMS
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PROTEINS
RADIOISOTOPES
RNA
TRACER TECHNIQUES
TRANSFERASES
VIRUSES
WASTES