Chromosome mapping of the human gene encoding the 68-kDa nuclear antigen (p68) by using the polymerase chain reaction

Journal Article · Tue Aug 01 04:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.16.6211· OSTI ID:7105642

Iggo, R; Gough, A; Lane, D P; Spurr, N K ^[1]; Xu, W ^[2]

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1. Imperial Cancer Research Fund Clare Hall Laboratories, Herts (England)
2. Imperial Cancer Research Fund, London (England)

Chromosome mapping by Southern analysis of DNA from somatic cell hybrids is often unsuccessful when only cDNA probes are available. p68 is a putative RNA helicase that is antigenically related to the simian virus 40 large tumor antigen. By using the polymerase chain reaction and oligodeoxynucleotide primers based on the cDNA sequence, the authors have identified introns in the p68 gene. Comparison of human and mouse DNA fragments amplified with these primers revealed length differences that allowed them to identify the human gene. Application of this technique to DNA from human-mouse somatic cell hybrids and cell lines derived from them by chromosome-mediated gene transfer allowed mapping of p68 to the distal part of long arm of chromosome 17.

• OSTI ID: 7105642
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:16; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHROMOSOMES
COLLAGEN
DAYS LIVING RADIOISOTOPES
DNA
DNA HYBRIDIZATION
DNA POLYMERASES
DNA SEQUENCING
ENZYMES
GENE AMPLIFICATION
GENES
GENETIC MAPPING
HETEROCHROMOSOMES
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MAPPING
MICE
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
POLYMERASES
PRIMATES
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
RODENTS
SCLEROPROTEINS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VERTEBRATES
VIRUSES